Dichroics > CoolLED

A Dichroic Mirror is required to combine different channels of light into a single optical path and is selected in each case to match the corresponding LED wavebands in the CoolLED illumination system.

When the LED wavebands are exchanged, it may be necessary to change the dichroic mirror at the same time depending on the new wavelengths installed in the system. This item is used within the pE-Collimator and the pE-Combiner.

 

The chart below shows the standard range of single LED wavelengths used in the pE-100 series of illumination systems.

Recent developments in LED technology have enabled increased intensity in what was historically the weaker green-yellow-red (“GYR”) region of the LED spectrum. A new wide spectrum GYR LAM is now supplied as standard in the pE-300^white and pE-4000 systems. It can be specified (or fitted retrospectively) in the pE-2 illumination systems.

Common LED wavelengths for live cell and fixed cell applications

LEDs only excite desired wavelengths explanation of benefits of discrete LED wavelengths

Why LEDs
Why LEDs for Life Sciences
Why CoolLED LEDs for Life Sciences

Why LEDs

LEDs (Light Emitting Diodes) are semiconductor devices which emit photons around a specific peak wavelength when an electrical current is passed through them. As semiconductors, they are:

• Fast switching
• Reliable
• Stable
• Long lasting

High light intensities are achievable. Although LEDs are around six times more efficient than conventional incandescent bulbs, they are still only 20 - 30% efficient. High brightness results in high heat flux densities so it is essential to provide the appropriate cooling to ensure that the LEDs do not overheat, resulting in spectral drift and premature failure.

Why LEDs for Life Sciences

The conventional illuminator for fluorescence was historically a high pressure mercury or metal halide bulb with multiple wavelength peaks creating a broad spectrum of “white” light. The majority of this light was unwanted and needed to be removed using optical filters. As individual LED peaks can be controlled independently, an LED illumination system can be operated so that only the desired wavelength peaks are illuminated for any particular stain(s) requiring excitation.

The benefits of LEDs can be considered from three aspects

Operational
Experimental
Environmental

• Instant on/off No shutters required, no warm up or cool down
• Simple to fit, simple to use no alignment, a once only adjustment
• Stable & repeatable reliable and consistent results
• Precise intensity control in 1% steps (0-100%) no ND filters required
• Excellent uniformity over field of view fixed and stable, no alignment necessary
• Long lifetime expected to exceed 25,000 hours of operating time
• Mercury-free
• Energy Efficient: 80% less power

Why CoolLED LEDs for Life Sciences

Only CoolLED uses active cooling of LEDs in all their illumination systems. As discussed above, LEDs are more efficient than conventional illuminators but still produce heat. By holding the LEDs at a constant temperature through thermal management of the LEDs, CoolLED can drive them more powerfully. This results in greater intensities. In addition, this increases stability, reliability, and lifetime of the LEDs.

It is CoolLED’s expertise in LED assembly and the use of active cooling which means that it can offer intensities up to five times greater than other LED illumination systems which use commercially available packaged LED parts.

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