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InVi SPIM Lattice Pro > LUXENDO

InVi SPIM Lattice Pro 요약정보 및 구매

The Inverted View Light-Sheet Microscopewith Advanced Illumination Module (AIM)

제조사 Luxendo
원산지 Germany
모델 InVi SPIM Lattice Pro
판매가격 전화문의
포인트 0점
배송비결제 주문시 결제

상품 정보

상품 기본설명

The Inverted View Light-Sheet Microscopewith Advanced Illumination Module (AIM)

상품 상세설명

The Advanced Illumination Module (AIM) is a modular extension of the InVi SPIM that provides the highest level of flexibility for illuminating your sample with a light-sheet.

It expands the capabilities of the InVi SPIM: while maintaining the ease-of-use and stability of the system, it adds tailorable, interactive adaptability of the beam shape to suit the highly specific requirements of your sample. You choose what gives you the best results for your 3D high resolution imaging experiment – large field of view, high speed or optimal spatial resolution. 

Illumination and Detection

The InVi SPIM Lattice Pro offers a variety of illumination patterns, ranging from the classical static Gaussian light-sheet or a scanned Gaussian beam to sophisticated illumination schemes like Bessel beams, Airy beams or optical lattices. 

The user can select from a broad choice of beam shapes to improve the microscope’s resolution and reduce photo-damage in delicate samples. A Special Optics 28.6x 0.7 NA water immersion objective lens projects the light-sheet on the sample. A high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens images the signal onto one or two Hamamatsu sCMOS cameras. An additional magnification changer provides 31.3x and 62.5x total magnification to allow you to optimize field of view and pixel size to your experimental needs. 



Effective Magnification 

Field of View 

Pixel Size 

 Optical Resolution


Nikon 25x/1.1NA 


420 µm 

208 nm

 255 nm


210 µm

104 nm


Illumination Optics

  • Chromatic correction from 440 to 660 nm
  • Light-sheet generation with static illumination or by beam scanning
  • Flexible light-sheet geometries: static and scanned Gaussian beams, Bessel and Airy beams and optical lattices for improved resolution, field of view and speed
  • Special Optics 28.6x 0.7 NA water immersion objective lens


Detection Optics

  • Nikon CFI Apo 25x W 1.1 NA water immersion objective lens
  • 2 spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
  • Filters adapted to the selected laser lines
  • 2 high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
  • Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
  • Peak quantum efficiency (QE): 82% @ 560 nm

InVi SPIM Lattice Pro: Beam Types 

The Gaussian beam


  • “Classic”, robust profile of well-known properties
  • High resolution, i.e., small beam waist


  • Divergent beam profile
  • Variable thickness across FOV
  • Short FOV at high resolution

The Bessel beam


  • Non-divergent beam profile
  • Long FOV
  • Highest resolution, constant across FOV
  • Combination with line detection mode removes side lobe artifacts


  • Side lobes of the Bessel beam carry as much energy as the core of the beam

The Optical Lattice
  • Multiple Bessel beams destructively interfering to cancel out a significant fraction of the side lobes 
  • Very high resolution over large FOV 
  • Robust against beam scattering
  • Illumination pattern is highly wavelength-dependent

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