InVi SPIM Lattice Pro > LUXENDO

The Inverted View Light-Sheet Microscopewith Advanced Illumination Module (AIM)

The Advanced Illumination Module (AIM) is a modular extension of the InVi SPIM that provides the highest level of flexibility for illuminating your sample with a light-sheet.

It expands the capabilities of the InVi SPIM: while maintaining the ease-of-use and stability of the system, it adds tailorable, interactive adaptability of the beam shape to suit the highly specific requirements of your sample. You choose what gives you the best results for your 3D high resolution imaging experiment – large field of view, high speed or optimal spatial resolution. 

Illumination and Detection

The InVi SPIM Lattice Pro offers a variety of illumination patterns, ranging from the classical static Gaussian light-sheet or a scanned Gaussian beam to sophisticated illumination schemes like Bessel beams, Airy beams or optical lattices. 

The user can select from a broad choice of beam shapes to improve the microscope’s resolution and reduce photo-damage in delicate samples. A Special Optics 28.6x 0.7 NA water immersion objective lens projects the light-sheet on the sample. A high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens images the signal onto one or two Hamamatsu sCMOS cameras. An additional magnification changer provides 31.3x and 62.5x total magnification to allow you to optimize field of view and pixel size to your experimental needs. 

Illumination	Detection	Effective Magnification	Field of View	Pixel Size	Optical Resolution
28.6x/0.7NA	Nikon 25x/1.1NA	31.3x	420 µm	208 nm	255 nm
		62.5x	210 µm	104 nm

 

Illumination Optics

• Chromatic correction from 440 to 660 nm
• Light-sheet generation with static illumination or by beam scanning
• Flexible light-sheet geometries: static and scanned Gaussian beams, Bessel and Airy beams and optical lattices for improved resolution, field of view and speed
• Special Optics 28.6x 0.7 NA water immersion objective lens

 

Detection Optics

• Nikon CFI Apo 25x W 1.1 NA water immersion objective lens
• 2 spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
• Filters adapted to the selected laser lines
• 2 high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
• Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
• Peak quantum efficiency (QE): 82% @ 560 nm

InVi SPIM Lattice Pro: Beam Types 

The Gaussian beam

Pro:

• “Classic”, robust profile of well-known properties
• High resolution, i.e., small beam waist

Con:

• Divergent beam profile
• Variable thickness across FOV
• Short FOV at high resolution

The Bessel beam

Pro:

• Non-divergent beam profile
• Long FOV
• Highest resolution, constant across FOV
• Combination with line detection mode removes side lobe artifacts

Con:

• Side lobes of the Bessel beam carry as much energy as the core of the beam

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