pE-4000 > CoolLED

Description

As the first company to introduce LEDs into fluorescence microscopy, CoolLED has developed a comprehensive understanding of the complexities of configuring and selecting filter sets for samples which have multiple stains.  These ’multi-band’ filters must have a corresponding absorption and emission band for each stain and the bands must be kept spectrally separate to ensure there is no overlap.

CoolLED’s innovation comes from recognizing that all the stains used in multi-band work can have their absorption bands divided into four separate groups across the spectrum due to the restricted bandwidth available.  This has allowed the development of a patent-pending, wavelength-grouping concept which makes it possible to deliver more power in an efficient four channel system.

With its unique 16 selectable LED wavelengths, the pE-4000 ensures optimum compatibility with all single and multi-band filter sets.  The flexibility and extensive functionality of the pE-4000 broadens the range of illumination options in core facilities.

DAPI/FITC/TRITC/Cy5 Quad filter excited by pE-4000 at matched wavelengths shown above

  The compact pE-4000 Light Source houses all the LEDs, their combining optics, thermal management and control electronics.  A dual mode control pod gives manual control in either White or Advanced modes.  Light is delivered to the microscope by either a single liquid light guide (LLG) or fiber. LLGs can be used in conjunction with CoolLED’s pE-Universal Collimator which can accept a microscope adaptor from CoolLED’s extensive range. For further information go to the Downloads tab

Features

The pE-4000 offers:

* Broadest LED spectrum available: 365-770nm match LED peaks to existing microscope filters

* Four channel wavelength-grouping design matches with all commercially available dual, triple, and quad filters

* Individual control of selected LED wavelengths less background, no filter wheel or shutters

* Integrated with many imaging software packages - can be incorporated into all experimental parameters

* Instant on/off no shutters required, no warm up or cool down

* Stable & repeatable reliable and consistent results

* Wide range of Microscope Adaptors fits most microscopes

* Precise intensity control in 1% steps (0-100%) no ND filters required

* Excellent uniformity over field of view fixed and stable, no alignment necessary

* Long lifetime expected to exceed 25,000 hours of operating time

IDEAL FOR MULTI-USER AND CORE FACILITIES

* Pre-sets allowing lab manager to match white spectrum to existing filter cubes

* Higher contrast images from matched-white spectrum

* Simple to fit, simple to use

* No training required

IDEAL FOR ADVANCED RESEARCH

* Individual LED wavelength selection

* Rapid switching between LED wavelengths enables capture of high speed events

* TTL & USB interfaces with imaging packages

* Excitation filters can be fitted in optical path for controlled switching with no moving parts

* Analogue inputs for dynamic intensity control

* Optical feedback for applications requiring higher stability

* Internal function generator for electrophysiology and optogenetics applications

Specification

Power values LED Wavelengths are divided across 4 channels with each channel having individual control.

Powers measured at sample plane of research grade microscope using 100% mirror in cube and 10X objectives. (Figures are typical values).  Note: wavelength settings 550nm and 580nm use single broad, high intensity peak (see spectrum under Description tab) Power requirements 110-240V a.c. 50/60Hz, 2.5A Power consumption Standby (i.e. no LEDs on) : Max 7W Single wavelength operation : Max 41W Dual wavelength operation : Max 75W Triple wavelength operation : Max 93W Quad wavelength operation : Max 112W Dimensions pE-4000 Light Source: 150mm(w) x 220mm(d) x 260mm(h) - Weight 3.5kg pE-4000 Control pod: 154mm(w) x 135mm(d) x 40mm(h) Weight 0.95kg pE-4000 Power Supply: 164mm(w) x 64mm(d) x 35mm(h) Weight 0.58kg pE-Expansion Box: 151mm(w) x 18mm(d) x 95mm(h) - Weight 0.34kg Environment & Safety LED products are more sustainable and energy efficient than conventional illuminators. CoolLED's products have the following benefits:

* Mercury-free

* Energy Efficient: 80% less power

* Long lifetime (25,000 operating hours)

* No bulb replacements

* Reduced risk of eye damage

* Quiet operation

* No special disposal regulations or issues

Warranty System = 12 months, LEDs = 36 months